Coding

Part:BBa_K3661001:Design

Designed by: Weixuan Lu   Group: iGEM20_CPU_CHINA   (2020-10-21)


Recombinant Human IL-22


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 187
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Mutation of CTG(10-12) to TTG,CTG(117-119) to TTG. We added the terminator TAA to the end of the sequence.


Source

Human genome,sequence found through Genbank https://www.ncbi.nlm.nih.gov/gene/50616

References